rabbit anti gad67 antibody  (Cell Signaling Technology Inc)

Journal: bioRxiv

Article Title: A selective top-down pathway from anterior cingulate cortex embeds a representation of saliency in hippocampus

doi: 10.1101/2025.06.26.661852

Figure Lengend Snippet: (A) Dual retrograde labeling of ACC neurons. Left: The injection sites of retrograde AAVs in RSCd, RSCg and dmSTR. Right: The distribution of fluorescently tagged neurons in the ACC. (B) The proportion of dmSTR-projecting, RSCd-projecting, RSCg-projecting ACC neurons located in ACC layer 2/3 or layer 5. (C) The fraction of ACC projection neurons which project to each area. (D) Example images of sections of ACC labeled with retrograde AAV injected in RSCd/g and immunostained with anti-CaMKIIα or anti-GAD67 antibodies. (E) Similar to (D) but injections of retrograde AAV were targeted to dmSTR. (F) The proportion of RSCd/g-projecting or dmSTR-projecting ACC neurons which were labeled with anti-CaMKIIα or anti-GAD67.

Article Snippet: Prior to staining, slices were blocked in blocking buffer (0.1 M Tris-HCl, 0.15 M NaCl, 0.5% (w/v) blocking reagent (41116100, Roche)) at room temperature (RT) for 1 h. Slices were then washed with TNB-T (0.1 M Tris-HCl (pH 7.5), 0.15 M NaCl, 0.5% (w/v) blocking reagent, 1% Triton (R) X-100) twice for 2 min each and incubated with a monoclonal mouse anti-CaMKIIα antibody (1:250; 6G9, Abcam), or a polyclonal rabbit anti-GAD67 antibody (1:300; GTX 101881), or a monoclonal rabbit anti-c-Fos antibody (1:1,000; 9F6 #2250, Cell Signaling) in TNB-T overnight at 4 °C.

Techniques: Labeling, Injection

Journal: Scientific reports

Article Title: Action inflexibility and compulsive-like behavior accompany neurobiological alterations in the anterior orbitofrontal cortex and associated striatal nuclei.

doi: 10.1038/s41598-024-84369-1

Figure Lengend Snippet: Fig. 4. Breeding mice for poor action flexibility and compulsive-like behavior results in changes in inhibitory synapse-associated protein levels in the VLO. (a) Coronal atlas image depicting the neuroanatomical location of the aMO and aVLO. (b) EB mice had higher levels of GAD67 in the aVLO. (c) GAD67 levels in aMO. did not differ between groups. (d) Meanwhile, in the aVLO, SLITRK3 was lower. (e) SLITRK3 levels did not differ in the aMO. (f) Coronal atlas image depicting the neuroanatomical location of the pMO and pVLO. (g) GAD67 levels were higher in the pVLO of EB mice and (h) in the pMO. (i) SLITRK3 levels did not differ between groups in the pVLO or (j) pMO. Ctrl refers to same-strain age-matched mice. Data shown as fold- change from control mean + SEM with circles representing individual mice. Ctrl n = 9–10, EB n = 9–11, with representative immunofluorescent images inset. Scale bar = 50 μm. *p ≤ 0.05, **p < 0.01.

Article Snippet: Primary antibodies were GAD67 (Cell Signaling 413182; 1:5,000), SLITRK3 (Sigma SAB3500965; 1:250), SAPAP3 (Invitrogen PA520465; 1:5,000), striatin (Novus NB110-74571; 1:250), and c-fos (Abcam 190289; 1:1,000).

Techniques: Control